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KMID : 1094720150200060980
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Biotechnology and Bioprocess Engineering
2015 Volume.20 No. 6 p.980 ~ p.987
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Functional characterization of Vibrio cholerae O1 WbeW enzyme responsible for initial reaction in O antigen biosynthesis
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Kim Chang-Sup
Seo Jeong-Hyun
Cha Hyung-Joon
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Abstract
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Vibrio cholerae O1 employs the ATP-binding cassette (ABC) transporter-dependent pathway for O antigen biosynthesis. Different from highly studied Klebsiella pneumoniae and Escherichia coli, it was reported that initial reaction of O antigen biosynthesis in V. cholerae O1 may be involved in WbeW protein, which is predicted to be a galactosyltransferase. In this work, we report expression and characterization of WbeW enzyme. WbeW was expressed as membrane-associated form in E. coli and it was obtained with high purity. The enzyme had a function of transferring Gal-1-P from UDP-Gal to Und-P, implying that initial glycan of O antigen in V. cholerae O1 can be composed of a Gal residue.
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KEYWORD
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WbeW enzyme, Vibrio cholerae O1, undecaprenyl phosphate galactose-1-phosphate transferase, membrane protein
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